Monthly Archives: June 2013

AK30 used for nanoparticle fabrication

Gullotti et. al. mixed PLGA with a PolyVivo AK30 (mPEG-PLGA 5000-4000, www.polyscitech.com) at a 75:25 ratio and processed via sonication-emulsion into nanoparticles for drug delivery.  When loaded with fluorescent PLGA-FITC the particles had an average size of 179.8nm with a zeta potential of -11.2. When loaded with paclitaxel the particles had an average size of 248.8nm and a zeta potential of -7.5 with a PTX load of 20.6% (w/w%). Find out more about their research here (http://link.springer.com/article/10.1007/s11095-013-1039-y)

AV11 used to stain nanoparticles

This is a great way to use our AV series, which could be helpful in your research! You can purchase the PolySciTech AV series and other polymers at our website:

https://akinainc.com/polyscitech/products/polyvivo/sequential.php

“Toxicity of surface-modified plga nanoparticles towards
lung alveolar epithelial cells”

Abstract
In vitro cytotoxicity and inflammatory response following exposure to nanoparticles (NPs) made of poly(lactide-co-glycolide) (PLGA) have been investigated on A549 human lung epithelial cells. Three different PLGA NPs (230 nm) were obtained using different stabilizers (polyvinyl alcohol, chitosan, or Pluronic® F68) to form respectively neutral, positively or negatively charged NPs. Polystyrene NPs were used as polymeric but non-biodegradable NPs, and titanium dioxide (anatase and rutile) as inorganic NPs, for comparison. Cytotoxicity was evaluated through mitochondrial activity as well as membrane integrity (lactate dehydrogenase release, trypan blue exclusion, propidium iodide staining). The cytotoxicity of PLGA-based and polystyrene NPs was lower or equivalent to the one observed after exposure to titanium dioxide NPs. The inflammatory response, evaluated through the release of the IL-6, IL-8, MCP-1, TNF-α cytokines, was low for all NPs. However, some differences were observed, especially for negative PLGA NPs that led to a higher inflammatory response, which can be correlated to a higher uptake of these NPs. Taken together, these results show that both coating of PLGA NPs and the nature of the core play a key role in cell response.

 

Grabowski, N., Hillaireau, H., Vergnaud, J., Aragao,
L.S., Kerdine-Romer, S., Pallardy, M., Tsapis, N., Fattal, E., TOXICITY
OF SURFACE-MODIFIED PLGA NANOPARTICLES TOWARDS LUNG
ALVEOLAR EPITHELIAL CELLS, International Journal of Pharmaceutics
(2013), http://dx.doi.org/10.1016/j.ijpharm.2013.05.025

AK24 used in Inner Ear Drug Delivery

You can purchase AK24 and other polymers with similar properties at
https://akinainc.com/polyscitech/products/polyvivo/sequential.php

Abstract

A convenient and efficient in vitro diffusion cell method to evaluate formulations for inner ear delivery via the intratympanic route is currently not available.The existing in vitro diffusion cell systems commonly used to evaluate drug formulations do not resemble the physical dimensions of the middle ear and round window membrane. The objectives of this study were to examine a modified in vitro diffusion cell system of a small diffusion area for studying sustained release formulations in inner ear drug delivery and to identify a formulation for sustained drug delivery to the inner ear. Four formulations and a control were examined in this study using cidofovir as the model drug. Drug release from the formulations in the modified diffusion cell system was slower than that in the conventional diffusion cell system due to the decrease in the diffusion surface area of the modified diffusion cell system. The modified diffusion cell system was able to show different drug release behaviors among the formulations and allowed formulation evaluation better than the conventional diffusion cell system. Among the formulations investigated, poly(lactic-co-glycolic acid)–poly(ethylene glycol)–poly(lactic co glycolic acid) triblock copolymer systems provided the longest sustained drug delivery, probably due to their rigid gel structures and/or polymer-to-cidofovir interactions.

http://informahealthcare.com/ddi

ISSN: 0363-9045 (print), 1520-5762 (electronic)
Drug Dev Ind Pharm, Early Online: 1–8
! 2013 Informa Healthcare USA, Inc. DOI: 10.3109/03639045.2013.789054

“Poly(lactic-co-glycolic) Acid−Controlled-Release Systems: Experimental and Modeling Insights”

ABSTRACT

Poly(lactic-co-glycolic acid) (PLGA) has been the most successful polymeric biomaterial used in controlled drug delivery systems. There are several different chemical and physical properties of PLGA that impact the release behavior of drugs from PLGA delivery devices. These properties must be considered and optimized in the formulation of drug release devices. Mathematical modeling is a useful tool for identifying, characterizing, and predicting mechanisms of controlled release. The advantages and limitations of poly(lactic-co-glycolic acid) for controlled release are reviewed, followed by a review of current approaches in controlled-release technology that utilize PLGA. Mathematical modeling applied toward controlled-release rates from PLGA-based devices also will be discussed to provide a complete picture of a state-of-the-art understanding of the control that can be achieved with this polymeric system, as well as the limitations.

The Rest of this article can be found in:
The Critical Review: The Drug Carrier System
DOI: 10.1615/CritRevTherDrugCarrierSyst.2013006475

pages 257-276
Volume 30, 2013

“Production, Sterilisation and Storage of Biodegradable Electrospun PLGA Membranes for Delivery of Limbal Stem Cells to the Cornea”

Abstract
Our aim was to produce, sterilize and store a synthetic, rapidly biodegrading membrane for cultured limbal stem cell transplantation. Membranes were electrospun from Poly(D,L-lactide-co-glycolide) with a 50:50 ratio of lactide and glycolide comparing 44 kg/mol and 153 kg/mol molecular weights (MW) and sterilized with γ-irradiation and stored for up to a year at a range of temperatures. Cells attached well on both MW membranes. The lower MW degraded faster than the higher MW membranes. γ-irradiation accelerated membrane breakdown when wet but sterilised membranes could be stored dry for at least a year at -20 °C.

 

To see the full research article refer to this website:
http://www.sciencedirect.com.ezproxy.lib.purdue.edu/science/article/pii/S1877705813010138

Procedia Engineering
PagesAhead of Print
Journal
CODEN:PERNBE
ISSN:1877-7058
DOI:10.1016/j.proeng.2013.05.099